General information
The SALSA MLPA
Probemix P476 ZNRF3 is a
research use only (RUO) assay for the detection of deletions or duplications in the
ZNRF3 gene.
ZNRF3 is an E3 ubiquitin-protein ligase that acts as a negative feedback regulator of Wnt signalling (Hao et al. 2012). Three independent studies show homozygous deletions of the
ZNRF3 gene in 10 to 16% of adrenocortical carcinoma cases (Assié et al. 2014; Juhlin et al. 2015; Zheng et al. 2016). Moreover, in 51% of microsatellite stable colorectal cancers deletion events at the
ZNRF3 locus are detected (Bond et al. 2016). Deletions of the
ZNRF3 gene are also found in osteoblastoma and prostate cancer (Nord et al. 2013; Robinson et al. 2015, Fraser et al. 2021).
This SALSA MLPA probemix is not CE/FDA registered for use in diagnostic procedures. Purchase of this product includes a limited license for research purposes.
Probemix content
The SALSA MLPA Probemix P476-A1 ZNRF3 contains 42 MLPA probes with amplification products between 130 and 433 nucleotides (nt). This includes 15 probes for the
ZNRF3 gene, plus four flanking probes for upstream genes and five flanking probes for downstream genes. In addition, 18 reference probes are included that target relatively copy number stable regions in various cancer types including adrenocortical carcinoma, colorectal cancer and prostate adenocarcinoma). Complete probe sequences and the identity of the genes detected by the reference probes are available in Table 3 and online (
www.mrcholland.com).
This probemix contains nine quality control fragments generating amplification products between 64 and 105 nt: four DNA Quantity fragments (Q-fragments), two DNA Denaturation fragments (D-fragments), one Benchmark fragment, and one chromosome X and one chromosome Y-specific fragment. More information on how to interpret observations on these control fragments can be found in the MLPA General Protocol and online at
www.mrcholland.com.