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Designing Synthetic Probes
General Questions on MLPA
What is MLPA?
What are the advantages of MLPA?
What are the limitations of MLPA?
Which equipment is recommended for MLPA analysis?
Is it possible to use MLPA to test the methylation status of a specific site?
Is it possible to detect point mutations with MLPA?
What is the difference between DNA and Methylation MLPA probes?
Is it possible to use SALSA MLPA probes on DNA from other organisms?
Which MLPA probemixes are CE certified?
Do I need to confirm MLPA results with other methods?
Can I have my samples tested at MRC-Holland?
Can I order synthetic probes from MRC-Holland?
What do the different version numbers mean?
Is MLPA a patented technology?
Why does MRC-Holland occasionally change the exon numbering or gene name used in its product materials?
Questions about the MLPA technique
To perform MLPA, is it necessary to get training?
Which quality controls are present in SALSA MLPA probemixes?
How many and what kind of control samples should be included in MLPA experiments?
What should a no DNA control look like in MLPA?
Does MRC-Holland have positive control samples available?
Is the purity and quality of the sample DNA important?
Is it possible to use DNA extracted from paraffin coupes for MLPA?
How important is the buffer used to dissolve the DNA?
Are SALSA MLPA reagents stable?
What are the unique features of the Ligase-65 enzyme?
Do mismatches close to the ligation site of probes influence results?
Is it possible to compare results from different experiments?
Why does the DNA MLPA protocol start with 5 min. incubation at 98 °C?
Can I decrease the duration of the hybridisation reaction?
Can I reduce the number of cycles in the PCR reaction?
Which factors influence relative probe signals?
Can I obtain the exact sequences recognised by the SALSA MLPA probes?
Is it possible to design a synthetic probe for a specific gene and add it to an existing SALSA MLPA probemix?
How can I analyse my MLPA data?
What can cause unexpected results in my MLPA peak patterns?
Ordering & payment information
How can I order from MRC-Holland?
How much are the shipping costs?
Do you have a distributor in my country?
If my country is part of the European Union, do I still have to pay VAT (TVA; MWS; BTW; IVA; MOMS)?
Is it possible to obtain a SALSA MLPA sample kit?
Is it possible to order a part of probemix/just one or more probes?
Can I order a probemix for less than 100 reactions?
Can I buy an extra vial of one of the buffers or the PCR primers from MRC-Holland?
Is it possible to order custom-designed probes?
What other products are available at MRC-Holland?
Are there any other licenses required for performing an MLPA reaction?
How do I pay?
When I received my order, all dry ice was gone. Will the MLPA kits be damaged?
Where can I find an SDS or MSDS for the products I ordered?
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